Incubation:Article Title: Detergent compositions and uses thereof
Article Snippet: This culture was then diluted (1:100) into 250 ml fresh medium (TSB+1% glucose) in a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap (Product #3293), and the flask was incubated at 37° C. for 2 days.
Article Title: Detergent compositions and uses thereof
Article Snippet: P. fluorescens was restreaked on TSA and incubated for 1 day at 20° C. The strain was inoculated into 10 mL of TSB and the culture was incubated statically for 16 hours at 20° C. After propagation, the culture was diluted (1:100) in 400 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4·7H2O, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine), added to a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap and incubated statically for 3 days at 20° C. The biofilm culture was subsequently pelleted by centrifugation (10 min, 8000 g, 25° C.), and the cells resuspended in 4 ml 3 M NaCl and incubated for 30 min at 30° C. to extract the surface-associated EPS.
Article Title: Detergent compositions and uses thereof
Article Snippet: P. fluorescens was restreaked on TSA and incubated for 1 day at 20° C. The strain was inoculated into 10 mL of TSB and the culture was incubated statically for 16 hours at 20° C. After propagation, the culture was diluted (1:100) in 400 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4.7H2O, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine), added to a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap and incubated statically for 3 days at 20° C. The biofilm culture was subsequently pelleted by centrifugation (10 min, 8000 g, 25° C.), and the cells resuspended in 4 ml 3M NaCl and incubated for 30 min at 30° C. to extract the surface-associated EPS.
Cell Culture:Article Title: Detergent compositions and uses thereof
Article Snippet: This culture was then diluted (1:100) into 250 ml fresh medium (TSB+1% glucose) in a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap (Product #3293), and the flask was incubated at 37° C. for 2 days.
Article Title: Detergent compositions and uses thereof
Article Snippet: P. fluorescens was restreaked on TSA and incubated for 1 day at 20° C. The strain was inoculated into 10 mL of TSB and the culture was incubated statically for 16 hours at 20° C. After propagation, the culture was diluted (1:100) in 400 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4·7H2O, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine), added to a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap and incubated statically for 3 days at 20° C. The biofilm culture was subsequently pelleted by centrifugation (10 min, 8000 g, 25° C.), and the cells resuspended in 4 ml 3 M NaCl and incubated for 30 min at 30° C. to extract the surface-associated EPS.
Article Title: Detergent compositions and uses thereof
Article Snippet: P. fluorescens was restreaked on TSA and incubated for 1 day at 20° C. The strain was inoculated into 10 mL of TSB and the culture was incubated statically for 16 hours at 20° C. After propagation, the culture was diluted (1:100) in 400 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4.7H2O, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine), added to a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap and incubated statically for 3 days at 20° C. The biofilm culture was subsequently pelleted by centrifugation (10 min, 8000 g, 25° C.), and the cells resuspended in 4 ml 3M NaCl and incubated for 30 min at 30° C. to extract the surface-associated EPS.
Centrifugation:Article Title: Detergent compositions and uses thereof
Article Snippet: This culture was then diluted (1:100) into 250 ml fresh medium (TSB+1% glucose) in a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap (Product #3293), and the flask was incubated at 37° C. for 2 days.
Article Title: Detergent compositions and uses thereof
Article Snippet: P. fluorescens was restreaked on TSA and incubated for 1 day at 20° C. The strain was inoculated into 10 mL of TSB and the culture was incubated statically for 16 hours at 20° C. After propagation, the culture was diluted (1:100) in 400 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4·7H2O, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine), added to a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap and incubated statically for 3 days at 20° C. The biofilm culture was subsequently pelleted by centrifugation (10 min, 8000 g, 25° C.), and the cells resuspended in 4 ml 3 M NaCl and incubated for 30 min at 30° C. to extract the surface-associated EPS.
Article Title: Detergent compositions and uses thereof
Article Snippet: P. fluorescens was restreaked on TSA and incubated for 1 day at 20° C. The strain was inoculated into 10 mL of TSB and the culture was incubated statically for 16 hours at 20° C. After propagation, the culture was diluted (1:100) in 400 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4.7H2O, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine), added to a Corning® CelIBIND® 225 cm2 Angled Neck Cell Culture Flasks with Vent Cap and incubated statically for 3 days at 20° C. The biofilm culture was subsequently pelleted by centrifugation (10 min, 8000 g, 25° C.), and the cells resuspended in 4 ml 3M NaCl and incubated for 30 min at 30° C. to extract the surface-associated EPS.
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